Morphology
Morphological analysis includes microscopical, histological and immunohistochemical methods using florescent, confocal microscopes and other relevant equipments.
Confocal microscopy is broadly used to resolve the detailed structure of specific objects within the cell. Similar to widefield fluorescence microscopy, various components of living and fixed cells or tissue sections can be specifically labeled using immunofluorescence, for example, and then visualized in high resolution. As a distinctive feature, confocal microscopy enables the creation of sharp images of the exact plane of focus, without any disturbing fluorescent light from the background or other regions of the specimen. Therefore, structures within thicker objects can be conveniently visualized using confocal microscopy. Furthermore, by stacking several images from different optical planes, 3D structures can be analyzed.
For more details see: Nikon A1R Simple Instruction Manual